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1. Theoretical part |
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I Cryobiological basis: from slow freezing to vitrification |
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II Vitrification of human oocytes and embryos |
The technical aspect |
Vitrification procedure
Concentration of CP: toxic or not?
Open vs closed carrier device: is there a difference?
Artifical Zona Hatching: helpful?
Effect of vitrification on chromosomal constitution?
Importance of warming
Handling of LN2
Security aspect of handling LN2
Cryo-Storage
Effect of long term storage in Open vs. Closed carrier devices
Tracability and identification of the straw
Control of the Temperature in degrees Celsius and level of LN2
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The biological aspect |
Vitrification of oocytes: the indications
Vitrification of zygotes: the indications
Vitrification of morulae: the indications
Vitrification of blastocysts: the indications (Vitrification on day 5 and day 6. Which one to select: the top blastocysts only? Blastocoele collapsing: a necessity?)
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Transfer of vitrified embryos |
How to prepare the patients?
Which embryo to select for ET?
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III The importance and indication of vitrification for mammalian embryos |
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2. Hands-On workshop |
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In this workshop, the most advanced protocol of vitrification for human oocytes and embryos will be demonstrated. |
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The vitrification and warming solutions will be explained in detail. |
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Each participant will have the opportunity to be individually trained for several hours on proper handling vitrification and warming of mice oocytes and embryos. |
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A detailed explanation of all the troubleshooting that may occur during the different phases of the vitrification, warming and storage phases is included. |